NRAS-BRAF Mutation Analysis by PCR

NRAS-BRAF Mutation Analysis by PCR Clinical Significance NRAS The NRAS proto-oncogene encodes a GTPase that functions in signal transduction and is a member of the RAS superfamily which also includes KRAS and HRAS. RAS proteins mediate the transmission of growth signals from the cell surface to the nucleus via the PI3K/AKT/MTOR and RAS/RAF/MEK/ERK pathways, which …

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BRAF Mutation Analysis by PCR

BRAF Mutation Analysis by PCR   Clinical Significance The BRAF gene encodes the B-Raf proto-oncogene serine/threonine kinase, a member of the RAF family of serine/threonine protein kinases which also includes ARAF and RAF1 (CRAF). BRAF is among the most commonly mutated kinases in cancer. Activation of the MAPK pathway occurs through BRAF mutations and leads …

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KRAS Mutation Analysis by PCR

KRAS Mutation Analysis by PCR Clinical Significance The KRAS proto-oncogene encodes a GTPase that functions in signal transduction and is a member of the RAS superfamily which also includes NRAS and HRAS. RAS proteins mediate the transmission of growth signals from the cell surface to the nucleus via the PI3K/AKT/MTOR and RAS/RAF/MEK/ERK pathways, which regulate …

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EGFR Mutation Analysis by PCR

EGFR Mutation Analysis by PCR Clinical Significance Recurrent somatic mutations in the tyrosine kinase domain ﴾TKD﴿ of EGFR are observed in approximately 10‐20% of lung adenocarcinoma, and at higher frequencies in never‐smoker, female, andAsian populations[PMID: 20388509; PMID: 25079552; PMID: 24071849; PMID: 22588877]. The most common mutations occur near the ATP‐binding pocket of the TKD and …

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